Analysis Note
Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m2/gPore volume (N2-isotherm): 0.74 - 0.84 ml/gd 50 (laser diffraction, size distribution): 5 - 7 µmLayer thickness: 150 - 200 µmDeviation of layer thickness per plate: ≤ 35 µmChromatographic testings: A) Cholesterin testhRf-values - cholesterin, cholesterin test: 11 - 23B) Steroid test - methyltestosterone, steroid test: 20 - 40 - Reichstein S, steroid test: 50 - 70 - hydrocortisone, steroid test: 65 - 85Typical value determined on an activated plate (120°C, 15 min) Eluent a) aceton/ water (95/5 v/v) Eluent b) acetonitrile/ water (70/30 v/v)
Application
Glass HPTLC Silica gel 60 RP-18 F254s plates. Silica gel HPTLC plates size 10 x 10 cm, 25 sheets.
General description
RP modified plates serve two purposes: act as a pilot method for HPLC and allow to choose various solvent system for special separations. Our RP-modified silica layers are well suited for many separation challenges that unmodified silica cannot overcome. These layers use aqueous solvent systems to separate extremely non-polar substances and analyze particular polar substances that can adapt to ion-pair chromatography. What's more, RP-modified silica layers are less dependent on atmospheric humidity. Unlike unmodified silica, RP-phases do not exhibit catalytic activity. This makes them the plates of choice for unstable substance that tend to experience oxidative degradation.
25 Glass plates 10 x 10 cm
Linkage
Replaces: 13724-5; 13724
Packaging
25 units in Fibre case
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